Colony counting for soft agar (3-D) mammalian cell assays
GelCount™ has been conceived specifically to detect, count and analyze stained or unstained, non-adherent mammalian cell colonies in soft agar ('3-D') assays. GelCount™ supports 6, 12, 24, 48 and 96-well plates as well as 35mm Petri dishes.
Applications:
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Key features
GelCount is unique automated system for high-throughput counting of cell colonies suspended in 3-D media such as soft agar and methylcellulose. By combining high-resolution/high depth-of-field scanning with innovative digital image processing, GelCount provides a cost effective solution to the difficult, subjective and labor intensive task of manually counting colonies suspended in media layers.
GelCount can simultaneously scan up to four multi-well plates for high through-put screening. GelCount’s advanced CHARM algorithm is capable of detecting both highly diffuse and overlapping colonies and is able to differentiate real colonies from biological debris, bubbles and staining artifact.
GelCount can rapidly detect and count both stained and unstained colonies down to 50 µm diameter.
- Cost Effective
GelCount is an automatic, easy-to-use and above-all cost-effective solution. GelCount relieves skilled technicians from the strain of laboriously counting cell colonies with expensive microscopes and automatic stages. - Objective
GelCount is both accurate and objective. GelCount eliminates ‘human error’ resulting from subjective interpretation, bias and technician fatigue. GelCount is consistent – effectively resolving the issue of judgment-induced errors that occur between different technicians and/or laboratories. - Stained or Unstained
GelCount can identify and count stained and un-stained colonies growing in most types of ‘3-D media’ such as soft agar and methyl cellulose. - High Capacity using Multi-Well Plates
GelCount can simultaneously scan up to four multi-well plates (6, 12, 24, 48 and 96) for high-throughput colony counting and screening. - High Resolution
GelCount can count individual colonies as small as 50 µm in diameter – stained or unstained. - Not Just Counting
GelCount’s feature-rich and easy-to-use software provides powerful multi-well plate navigation and preview functions and delivers a range of unique statistical outputs including colony diameter, volume and optical density histograms. GelCount output is Microsoft® Excel® compatible.
For more information please contact: sales@discovtech.com
